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KMID : 0387720110220020099
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Korean Journal of Blood Transfusion
2011 Volume.22 No. 2 p.99 ~ p.109
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Comparison of Methods for Detecting Bacterial Contamination in Platelet Concentrates
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Kwon So-Yong
Choi Kyoung-Young
Lim A-Hyun
Park Kyoung-Un
Cho Nam-Sun
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Abstract
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Background:Bacterial contamination of platelets represents the highest infectious risk for a transfusion. In this study, we evaluated 2 culture-based systems that have been approved by the US FDA for bacterial screening.
Methods: Platelet concentrates were inoculated with 5 bacterial species to give a final concentration of 100, 101 and 102 CFU/mL. Samples for culture were taken immediately after inoculation (0 hr sample) and after 24 hrs (24 hr sample). For the BacT/ALERT 3D system, a 10 mL sample was inoculated into an aerobic culture bottle and incubated for 7 days. For the Pall eBDS system, 3 mL samples were taken from the 0 hr and 24 hr samples, respectively. The samples were incubated for 24 hrs and 30 hrs.
Results: Both systems detected all inoculated units both in the 0 hr and 24 hr samples, except for units inoculated with K. pneumoniae. Eleven units out of 30 units inoculated with K. pneumoniae were detected by the BacT/ALERT 3D system in the 24 hr samples. The Pall eBDS system detected 8 of 30 units in the 24 hr samples.
Conclusion:Implementation of either system will decrease the risk of transfusing bacterially contaminated platelets. However, testing for bacterial contamination will not completely prevent septic transfusion reactions; pathogen inactivation that is now available should also be considered as an alternative method to reduce the risk of bacterial contamination. (Korean J Blood Transfus 2011;22:99-109)
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KEYWORD
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Bacterial contamination, Platelets, BacT/ALERT 3D system, Pall eBDS system
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